Mechanisms of Antiproliferative Action of Streptococcal Arginine Deiminase Against Jurkat Lymphoblastic Leukemia Cells

Abstract

Arginine deprivation strategy is considered as a promising trend in cancer therapy. The aim of the study was to investigate the influence of streptococcal arginine deiminase on Jurkat lymphoblastic leukemia cells. The effects of the supernatants of the destroyed streptococci of the original strain expressing arginine deiminase and its isogenic mutant with the inactivated arcA gene were compared. Cell proliferation was evaluated in an MTT-test. The remaining parameters were examined using flow cytometry. The cell cy-cle changes were studied using DAPI dye and anti-cyclin A2 antibodies. The autophagy intensity was assessed using the LysoTracker ™ Green DND-26 reagent. To investigate cell viability DAPI staining was performed. Streptococcal arginine deiminase suppressed proliferative activity Jurkat lymphoblastic leukemia cells, increased the proportion of cells in the G0/G1 phases, reduced S/G2 phases proportion of cells and enhanced autophagy, without declaing viability. Arginine supplementation leveled the effects of the enzyme. The obtained results open up the possibility of using arginine-hydrolyzing activity of the streptococcal enzyme for combined therapy of oncological diseases.