Abstract
Listeria monocytogenes was injured by treating the bacterium with a solution of 1% (w / v) potassium sorbate for 30 min. Injury was shown by inability of the bacterium to tolerate 6% NaC1 in tryptose agar (TA) and ability to grow on TA with no added salt. Colony-forming ability of injured cells was restored when they recovered in tryptose broth containing sub-lethal amounts of metabolic or synthetic inhibitors. 2,4-Dinitrophenol was the only inhibitor which effectively prevented recovery of injured cells. No changes occurred in the cell membrane that allowed soluble proteins or nucleotides to leak from cells during the course of sorbate injury.
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