Abstract
EZH2 is an important target in a variety of tumors, but its role in KIRP (Kidney renal papillary cell carcinoma) has not yet been proven. The aim of this study was to investigate the role of EZH2 in KIRP patients. And to investigate the KIRP, we used two KIRP cell lines, SKRC39 and ACHN in this study. We used CCK8 assay and colony formation assay to study the effect of EHZ2 small molecule inhibitor EPZ6438 on cancer cell proliferation, we used Trypan Blue cell counting experiments to study the effect of EZH2 on cancer cell apoptosis, and we also used qRT-PCR to study the mechanism of EZH2 on cancer cell proliferation. The results showed that EZH2 was elevated in KIRP patients; inhibition of EZH2 could inhibit the proliferation of cancer cells and induce the apoptosis of cancer cells, which is valuable for the control of KIRP in patients. As for the mechanism, we found that inhibition of EZH2 upregulated P53 expression and thus activated this tumor suppressor gene, indicating why EHZ1 inhibits renal cancer cells.
Highlights
Renal cancer accounts for 2% to 3% of adult malignancies and accounts for 80% to 90% of adult renal malignancies
We used CCK8 assay and colony formation assay to study the effect of EHZ2 small molecule inhibitor EPZ6438 on cancer cell proliferation, we used Trypan Blue cell counting experiments to study the effect of EZH2 on cancer cell apoptosis, and we used qRT-PCR to study the mechanism of EZH2 on cancer cell proliferation
The results showed that EZH2 was elevated in Kidney renal papillary cell carcinoma (KIRP) patients; inhibition of EZH2 could inhibit the proliferation of cancer cells and induce the apoptosis of cancer cells, which is valuable for the control of KIRP in patients
Summary
Renal cancer accounts for 2% to 3% of adult malignancies and accounts for 80% to 90% of adult renal malignancies. The incidence rates in developed countries are higher than those in developing countries. The incidence rates in urban areas are higher than those in rural areas. The incidence rates in males are more than in females. Kidney renal papillary cell carcinoma (KIRP) is one kind of kidney cancer [2]. Kidney renal papillary cell carcinoma is the cancer of tubes that filter those waste products from the blood. There are two types of papillary renal cell carcinoma. Type 1 is more common and grows slowly. KIRP makes up about 15% of all renal cell carcinoma, which is the most common type of kidney cancer. EPZ6438 is an effective, highly selective inhibitor of EZH2 methyltransferase with an IC50 of 9.9 nM and more than 1000-fold higher selectivity for EZH2 than the other 20 human methyltransferases
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