Abstract
This work present a study of the zinc oxide nanocomposites intracellular synthesis by lactic acid bacteria. The kinetic processes on the ZnO biocolloids interface are described as well. The kinetic data showed that the process of ZnO NPs formation is expressed with an initial rapid stage and after that the system undergoes to the equilibrium with the efficiency of the process of about 85 ± 1.59%. The biosynthesis has been studied by a number of the instrumental methods such as the spectroscopy approach (ultraviolet-visible and Fourier transform infrared spectroscopy), the spectrofluorometric analysis, the scanning and transmission electron microscopy with an energy dispersive X-ray. Furthermore, the antibacterial activity of ZnO NPs against two, Gram(+) and Gram(-), clinically relevant drug-resistant strains by using the fluorescence microscopy was tested. Furthermore, the obtained results allow us to describe, for the first time, the intracellular ZnO NPs biosynthesis mechanism. It was reported that carboxyl functional group are mostly involved in ZnO NPs formation.
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