Abstract
Coliphage T7 suspended in protective media are inactivated by X-rays more rapidly when the DNA contains 5-bromouracil (5-BU) replacing thymine. The percentage of the total number of inactivated phages which have been killed by the production of double-strand breaks in the DNA has been measured by means of an ultracentrifugal technique which yields the ratio of broken to unbroken DNA molecules. It was found that the sensitization by 5-BU substitution results only partially from an increase in the rate of strand breakage. If the phage are irradiated in a N 2 atmosphere and in the presence of cysteine, all non-breakage damage is eliminated. From this it is hypothesized that 5-BU phage are more sensitive than normal phage because 5-BU is more easily damaged than thymine.
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