Mechanism of sugar transport and phosphorylation via permeases of the bacterial phosphotransferase system: Catalytic residues in the β-glucoside-specific permease as defined by site-specific mutagenesis

Abstract

The mechanism of action of the β-glucoside permease (II bgl) of the Escherichia coli phosphotransferase system (PTS) was investigated employing site-specific mutagenesis and a variety of kinetic approaches. The enzyme catalyses three phosphoryl transfer reactions: a) phosphorylation of site 1 in the C-terminal domain with phospho-