Mechanism of Sang-Ju-Yin on Hand, Foot and Mouth Disease Based on Network Pharmacology

Abstract

<i>Objective</i>: Hand, foot and mouth disease (HFMD) is an acute infectious disease caused by enterovirus 71 (EV71), Coxsackie virus A16 (CA16) and other enteroviruses. For the treatment of HFMD, there are no recognized specific treatment drugs. In recent years, with the continuous development of traditional Chinese medicine, the clinical use of traditional Chinese medicine in the treatment of HFMD reports is increasing. At present, traditional Chinese medicine is mainly used to disperse wind and heat, clear heat and detoxify, clearing damp and dispel evil, but most of them do not know the exact mechanism of treatment. In this paper, the network pharmacology method was adopted to analyze the main active components and action targets of Sang-Ju-Yin (SJY) and to construct corresponding pathways, and to explore the mechanism of action of SJY in the treatment of HFMD. <i>Methods</i>: The active components of SJY were collected and potential targets were searched by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). GeneCards platform was used to find disease targets, and a protein interaction network (PPI) was constructed using the STRING platform. Cytoscape 3.6.0 software was used to screen out the key targets. The enrichment analysis of KEGG pathway and gene function analysis (go) was carried out by R language bioconducor package. <i>Results</i>: There were 151 main active components such as quercetin, luteolin and wogonin, and 15 intersection targets were obtained after 78 targets and 100 hand foot mouth disease targets intersected. Key pathways such as TNF signaling pathway, measures and influenza A were obtained by KEGG analysis. <i>Conclusions</i>: The main active components of SJY are quercetin, luteolin, wogonin, kaempferol, aloe emodin, Licochalcone A. It mainly regulates AKT1, Bax, IKBKB, IL-6, STAT3 and other targets, regulates TNF, influenza A and other signaling pathways to inhibit inflammatory response and regulate immune function, so as to achieve the purpose of treating hand foot mouth disease.