Mechanism of Receptor-Interacting Protein 3 on Tumor Necrosis Factor Alpha in Gallbladder Cancer Cells

Abstract

To investigate the mechanism of receptor-interacting protein 3 on the proliferation, invasion and reactive oxygen species levels of gallbladder cancer cells induced by tumor necrosis factor alpha. Gallbladder cancer SGC-996 cells were divided into a control group, a plasmid cloning deoxyribonucleic acid-negative control group, a plasmid cloning deoxyribonucleic acid-receptor-interacting protein 3 group, a plasmid cloning deoxyribonucleic acid-receptor-interacting protein 3+tumor necrosis factor alpha group and a plasmid cloning deoxyribonucleic acid-receptor-interacting protein 3+si-receptor-interacting protein 3 group. Reverse transcription polymerase chain reaction for messenger ribonucleic acid expression in cells; detection of cell proliferation, invasion and reactive oxygen species in cells by cell counting kit-8, Transwell and flow cytometry, respectively; detection of receptor-interacting protein 3, matrix metallopeptidase-9, matrix metallopeptidase-2 proteins in cells (Western blotting method). The expression of receptor-interacting protein 3 messenger ribonucleic acid was lower in gallbladder cancer cells than in gallbladder epithelial cells (p<0.05) and lowest in SGC-996 cells (p<0.05). receptor-interacting protein 3 messenger ribonucleic acid, cell survival rate, invasion ability, reactive oxygen species level, receptorinteracting protein 3, matrix metallopeptidase-2 and matrix metallopeptidase-9 protein were similar in the control and plasmid cloning deoxyribonucleic acid-negative control groups (p>0.05), while receptorinteracting protein 3 messenger ribonucleic acid, receptor-interacting protein 3 protein, reactive oxygen species content were elevated. And cell survival, invasion ability, matrix metallopeptidase-2 and matrix metallopeptidase-9 protein were decreased in the plasmid cloning deoxyribonucleic acid-receptorinteracting protein 3 group (p<0.05). Compared with the plasmid cloning deoxyribonucleic acid-receptorinteracting protein 3 group, receptor-interacting protein 3 protein, reactive oxygen species content were increased and cell survival, invasion ability, matrix metallopeptidase-2 and matrix metallopeptidase-9 protein were decreased in the plasmid cloning deoxyribonucleic acid-receptor-interacting protein 3+tumor necrosis factor alpha group and receptor-interacting protein 3 protein, reactive oxygen species content were decreased and cell survival, invasion ability, matrix metallopeptidase-2 and matrix metallopeptidase-9 protein were increased in the plasmid cloning deoxyribonucleic acid-receptor-interacting protein 3+sireceptor interacting protein 3 group (p<0.05). Overexpression of receptor-interacting protein 3 inhibited tumor necrosis factor alpha induced proliferation and invasion of gallbladder cancer cells and promoted reactive oxygen species levels in tumor necrosis factor alpha induced gallbladder cancer cells and its mechanism was related to matrix metallopeptidase-2 and matrix metallopeptidase-9 protein expression.