Abstract
Application of plant growth promoting rhizobacteria (PGPR) to boost up production and reduce pathogenesis is an imminent approach in agriculture. One of the PGPR (RR18) was found to have best plant growth promoting characteristics among 41 PGPR shortlisted from the 162 bacteria isolated from the groundnut rhizosphere. RR18 reduced disease incidence and increased germination of groundnut seeds in presence of A. niger. RR18 was found to induce systemic resistance in A. hypogaea L. as it induced chitinase, ß – 1,3- glucanase, phenylalanine ammonia lyase, peroxidase, polyphenol oxidase, lipoxygenase and jasmonic acid. Investigation of the specific mechanism by a comparative 2D gel electrophoresis of bacteria treated and untreated groundnut seedlings revealed induction of several proteins, two of them very prominently seen were- 60 kD and 56 kD proteins. These proteins were identified as Phospho-2-dehydro-3-deoxyheptonate aldolase 1 and auxilin related protein 1 using MALDI TOF and Mascot analysis. Phospho-2-dehydro-3-deoxyheptonate aldolase 1 is reported to synthesize secondary metabolites derived from intermediates of the pre-chorismate pathway up to shikimate. Auxilin related protein 1 belongs to DnaJ protein family and reported to be involved in protein folding, protein transport and plant growth promoting activity. 16S rDNA sequence of RR18 showed 99 % homology to Burkholderia species.
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