Abstract
Fundamental studies on the availability of oxygen from the decomposition of H(2)O(2), in vivo, by Xanthomonas campestris, when H(2)O(2) is used as an oxygen source are presented. It was found that the H(2)O(2) added extracellularly (0.1-6 mM) was decomposed intracellularly. Further, when H(2)O(2) was added, the flux of H(2)O(2) into the cell, is regulated by the cell. The steady-state H(2)O(2) flux into the cell was estimated to be 9.7 x 10(-8) mol m(-2) s(-1). In addition, it was proved that the regulation of H(2)O(2) flux was coupled to the protonmotive force (PMF) using experiments with the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP), which disrupts PMF. The coupling constant between the rate of free energy availability from PMF and the rate of reduction of H(2)O(2) flux, was found to be 46.4 mol m(-2) s(-1) J(-1) from simulations using a developed model. Also, the estimated periplasmic catalase concentration was 1.4 x 10(-9) M.
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