Abstract
Non-small cell lung cancer (NSCLC) is regarded as one of the major intractable diseases, which was cured mainly by chemotherapeutics in the clinical treatment at present. But it is still a vital mission for the current medical and researchers that hunting a natural medicine which have little side effects and high-efficiency against the NSCLC on account of the shortcomings on current drugs. Nepeta cataria L. plays an important role in anti-cancer treatment according to the reports which was recorded in the Chinese Pharmacopoeia of version 2015 and belongs to one of the Traditional Chinese medicine (TCM). Microfluidic chip technology is widely used in scientific research field due to its high-throughput, high sensitivity and low cost with the continuous progress of science and technology. In this study, we investigate the effect of total flavonoid extracted from Nepeta cataria L. (TFS) through human lung cancer cell line A549 based on the microfluidic device and Flow Cytometry. So we detected the mRNA expression of MicroRNA-126 (miR-126), VEGF, PI3K, PTEN and proteins expression respectively to explore the partial PI3K-AKT pathway molecular mechanisms through Quantitative Real-time PCR (qRT-PCR) and Western Blot. The results showed that TFS can disturb the expression of miR-126 and regulate the PI3K-AKT signaling pathway to meet the effect of anti-cancer. Taking all these results into consideration we can draw a conclusion that TFS may be used as a novel therapeutic agent for NSCLC in the near future.
Highlights
Lung cancer is one of the major diseases which were seriously endangering human’s health and life according to related data statistics, the death rate of cancer presents an increasing trend year by year, especially Non-small cell lung cancer (NSCLC) which was the most common type of lung cancer
We investigate the effect of total flavonoid extracted from Nepeta cataria L. (TFS) through human lung cancer cell line A549 based on the microfluidic device and Flow Cytometry
The results showed that TFS can disturb the expression of miR-126 and regulate the PI3K-AKT signaling pathway to meet the effect of anti-cancer
Summary
Lung cancer is one of the major diseases which were seriously endangering human’s health and life according to related data statistics, the death rate of cancer presents an increasing trend year by year, especially NSCLC which was the most common type of lung cancer. (family, Limiaceae; order, Lamiales), is one of the TCM which was found in the Eastern Mediterranean, Southern Asia and China [3,4,5]. It is distributed all round the world, for example north, northwest, northeast and west, commonly used in local people’s daily life. From the literature investigation we can see that the volatile oil and flavonoid play important roles in many pharmacological actions, such as anti-cancer including breast cancer and prostatic cancer. Nepeta cataria L. is a promising drug for anti-cancer agent. The mechanism of anti-cancer action has not been understood absolutely
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