Mechanism of miR-214 on the proliferation and apoptosis of oropharynx squamous cell carcinoma cell line by regulating Notch1 signal

Abstract

Objective To study the mechanism of miR-214 on the proliferation, invasion and apoptosis of oropharyngeal squamous cell carcinoma (OSCC) cell line by regulating Notch 1 signal. Methods OSCC cell lines were purchased from ATCC cell bank and divided into three groups: inhibition group (IN group) , simulation group (SI group) and control group (CO group) . Notch 1 protein, mRNA expression, apoptosis, migration and proliferation were analyzed by Western blot, qRT-PCR, TUNEL, invasion experiment and CCK-8. One way ANOVA was used to compare differences among the three groups, LSD-t test was used for comparison between two groups. Result The expression of mir-214m RNA in the cells of IN group, SI group and CO group were (1.15 ±0.26) , (3.34±0.89) , (2.08±0.67) respectively, and there was significant difference between the SI group and IN group, SI group and CO group (t = 5.281, P = 0.002; t = 2.529, P = 0.045) ; The apoptosis rates of OSCC cells in the three groups were (0.78±0.10) ﹪, (0.32±0.06) ﹪, (0.56±0.12) ﹪, respectively. The difference between IN group and CO group, IN group and SI group were statistically significant (t = 3.149, P = 0.020; t = 8.823, P < 0.001) ; The proliferation rates of cancer cells in the three groups were: (0.65±0.05) ﹪, (1.26±0.06) ﹪, (1.89±0.04) ﹪, respectively. The difference between IN group and SI group, IN group and CO group were statistically significant (t = 11.056, P < 0.001; t = 27.394, P < 0.001) . The difference between CO group and SI group was statistically significant (t = 12.365, P < 0.001) ; the invasive rates of cancer cells in the three groups were: (0.13±0.02) ﹪, (0.89±0.13) ﹪, (0.48±0.11) ﹪, respectively. The difference between SI group and CO group, SI group and IN group were tatistically significant (t = 4.176, P = 0.006; t = 10.147, P < 0.001) ; The levels of Notch1 protein in the three groups were (2.38±1.34) , (0.94 ± 0.23) , (1.76 ± 0.67) , respectively. There was a significant difference between SI group and CO group (t = 2.588, P = 0.041; t = 2.368, P = 0.056) ; The expression of Notch1 mRNA in the three groups were (4.26 ± 1.06) , (1.45 ± 0.38) , (2.46 ± 0.87) , respectively. The difference was statistically significant (t = 2.935, P = 0.026; t = 5.583, P = 0.001) . Conclusion miR-214 levels are elevated and may be inversely related to the proliferation of OSCC cell lines. MiR-214 inhibits Notch1 signaling pathway expression, promotes growth and invasion of cancer cells, and inhibites apoptosis of cancer cells. Key words: miR-214; Notch1; Oropharyngeal squamous cell carcinoma