Abstract
A lipid peroxidation reaction and methylene blue bleaching reaction are thought to proceed in the presence of methylene blue with linoleic acid as the substrate from the time lipoxygenase is added until methylene blue bleaching concludes. For 15 s after adding the lipoxygenase, neither the lipid peroxidation reaction nor the bleaching reaction occurred, but they both proceeded after that time period. The results suggested that hydroperoxide is first formed by the lipid peroxidation reaction, after which methylene blue specifically picks the hydrogen from the hydroperoxide 13-OOH isomers, and then the methylene blue is reduced.
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