Abstract
Purpose: To observe the intervention effect of isoquercitrin on related factors in PERK-eIF2a-ATF4 signaling pathway after it acts on HepG2 cells. Materials and Method: HepG2 cells were tested with different concentrations of isoquercitrin. MTT assay was employed to detect the cell proliferation. Expressions of GRP78, PERK, elF2a, ATF4 and CHOP mRNA and protein were measured by qRT-PCR and Western blot. Results: Cell experiments showed that isoquercitrin inhibited the proliferation of HepG2 cells in a dose-and time-dependent manner. Compared with the control group, the expressions of GRP78, PERK, elF2a, ATF4, CHOP mRNA and the expressions of GRP78, p-PERK, elF2a, ATF4, CHOP protein were significantly increased after isoquercitrin treatment, and the expressions of PERK protein decreased significantly. Conclusion: The present study verified that isoquercitrin induce the apoptosis of HepG2 cells, and its mechanism may be related to the intervention of related factors in PERK-eIF2a-ATF4 signaling pathway.
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