Abstract
The effect of interferon pretreatment of monkey kidney CV-1 cells on the accumulation and stability of simian virus-40 (SV 40) early RNA was examined using the temperature-sensitive mutant, tsA 30, in the presence of cytosine arabinoside. Interferon reduced the accumulation of SV 40 early RNA by equivalent amounts in both the nucleus and cytoplasm. In pulse-chase experiments about 80% of the SV 40 early RNA decayed with a half-life of about 4 hr; the remaining 20% decayed much more slowly. The decay rate of SV 40 early RNA was indistinguishable between interferon-treated and untreated cells in both nuclear and cytoplasmic fractions. These results suggest that interferon pretreatment of monkey kidney cells mediated a reduction in synthesis rather than an enhancement in degradation of SV 40 early RNA.
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