Mechanism of inhibition of Chromatium D growth by L-methionine regulation of L-threonine biosynthesis by the intracellular level of S-adenosylmethionine

Abstract

1. (1) An unusual accumulation of S- adenosyl- L-methionine in Chromatium D was associated with a marked growth inhibition by L-methionine. The inhibition was overcome by L-isoleucine, L-leucine, L-phenylalanine, L-threonine, L-valine and putrescine. Based on their effects, these compounds are classified into 3 types. 2. (2) L-Isoleucine, L-leucine, L-phenylalanine and L-valine (Type I) inhibited the L-methionine uptake and consequently prevented the bacterium from the unusual accumulation of S- adenosyl- L-methionine even in the presence of L-methionine in the medium. Putrescine (Type II) stimulated the consumption of S- adenosyl- L-methionine , but did not influence the L-methionine uptake. Hence, the effect of putrescine would be explained by the action to diminish the intracellular level of S- adenosyl- L-methionine . L-Threonine (Type III) neither inhibited the L-methionine uptake nor affected the content of S- adenosyl- L-methionine due to the addition of L-methionine. 3. (3) The specific activity of homoserine kinase (EC 2.7.1.39) was greatly lowered by the addition of L-methionine under conditions in which Chromatium D unusually accumulates S- adenosyl- L-methionine . Homoserine dehydrogenase (EC 1.1.1.3) activity was inhibited by S- adenosyl- L-methionine (50% inhibition index, 3.5 mM). These facts strongly suggest that the growth inhibition by L-methionine is associated with the L-threonine deficiency caused by the unusual accumulation of S- adenosyl- L-methionine .