Abstract

The inhibition of cellular DNA synthesis that occurs after infection of rabbit kidney cells with pseudorabies virus is not due to the successful competition of viral DNA with cellular DNA to act as a template for DNA replication. It is also not due to a greater affinity of the DNA polymerase present in virus-infected cells for viral DNA than for cellular DNA. The inhibitory process is arrested by the addition of puromycin to the infected cells. It is concluded that a protein is responsible for the inhibition of the synthesis of cellular DNA in the infected cell.

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