Abstract
It was to investigate the role of Gegen Qinlian decoction (GQD) in the regulation of ABTB1 gene based on PI3K/AKT/FOXO1 signaling pathway in colorectal cancer (CRC) metastasis. In this study, 10 cases of the CRC mouse model were established by inoculating CT26 cells into the spleen of mice, which were divided into the experimental group and the control group, 5 cases in each group; the control group was intragastrically administered with normal saline 0.3 mL/d, and the experimental group was intragastrically administered with GQD 0.2 mL/d at a ratio of 0.2 g medicinal materials/10 g for 10 days and sacrificed, and pathological sections were made. The expression density of signaling pathway PI3K/AKT/FOXO1 as well as gene ABTB1 was detected in the sections of the two groups, and the mechanism of action of this gene in the two groups of mice was studied. It was found that the densities of p-PI3K, p-AKT, and p-FOXO1 in the experimental group of mice were 26.55 g/cm3, 70.2 g/cm3, and 24.36 g/cm3, respectively, which were significantly increased compared with the control group, P < 0.05; the density of ABTB1 was 35.4 g/cm3, which was significantly increased compared with the control group, P < 0.05; the proliferation and migration ability of CRC cells in the experimental group were significantly decreased, P < 0.05. GQD can promote the expression of ABTB1 by activating the PI3K/AKT/FOXO1 signaling pathway, in order to inhibit the proliferation and growth ability of CRC cells.
Highlights
Colorectal cancer (CRC) is a common malignant tumor in the gastrointestinal tract
It was reported in the literature that Gegen Qinlian decoction (GQD) can successfully express ABTB1 by activating Phosphatidylinositol 3-kinase (PI3K)/AKT/FOXO1 signaling pathway and inhibit the proliferation and growth ability of CRC cells, which is consistent with the results of this study [15]
The results of this study showed that the positive expression rate of PI3K in CRC was 60%, and the expression of PI3K was significantly correlated with the depth of tumor invasion, differentiation, and lymph node metastasis
Summary
The incidence of CRC in males and females ranks the third and second most common malignant tumors, respectively, and its incidence is increasing to varying degrees in most countries in the world [1]. With the continuous improvement of people’s living standards and changes in dietary habits, the incidence of CRC in China has shown a significant increase in many areas [2]. The disease is more common in middle-aged men, most common between 40 and 70 years of age, with an incidence of about 2 : 1 in men and women It can occur in any part of the colon or rectum, with the rectum and sigmoid colon being the most common, followed by the cecum, ascending colon, descending colon, and transverse colon [3]. How to improve the therapeutic effect and survival rate of CRC has been the goal of scientists’ research
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