Abstract
The block copolymer Pluronic F127 and water form a gel-like cubic phase which is useful as sieving media for electrophoretic separation of DNA fragments in capillary electrophoresis because, below a transition temperature of 11 °C, the gel melts to an easily handled liquid solution. Here we study the conformational state and the mode of electrophoretic migration of double stranded DNA in the cubic gel. The DNA remains in B form in the Pluronic F127 gel and functions as a substrate for PCR, and there is no detectable effect on the Pluronic solution−gel transition temperature by the presence of DNA of concentrations up to the overlap concentration. Velocity and coil deformation measurements during electrophoresis show that the mode of DNA migration in this gel is qualitatively different compared to conventional gels. DNA coils are deformed with the helix axis preferentially perpendicular to the field direction, and the electrophoretic mobility varies discontinuously with field strength. At low field strengt...
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