Mechanism of cisplatin combined with zoledronic acid on lung cancer A549 cells

Abstract

Objective To investigate the function of cisplatin combined with Zoledronic acid on proliferation and mechanisms of A549 cells.Methods ( 1 ) Methyl thiazol tetrazolium (MTT) assay and Annexin V-FITC/PI double-staining flow cytometry were employed to observe the effects of cisplatin combined with Zoledronic acid upon anti-proliferation and apoptosis respectively.Reverse transcription-polymerase chain reaction (RT-PCR) was applied to assay mRNA expression of MDC1 among different groups.Results Inhibition of the cell proliferation was observed under the treatment of combined cisplatin and zoledronic acid (39.16 ±4.94)％,superior to the treatment of zoledronic acid ( 19.66 ±4.57)％ or cisplatin ( 16.87 ± 2.50) ％.Combined group induced A549 cells apoptosis ( 32.30 ± O.50 ) ％,compared with cisplatin (23.90 ± 2.46) ％,zoledronic acid ( 18.87 ± 3.04 ) ％,the difference was statistically significant; cisplatin after zoledronic acid treatment of A549 cells M DC1 mRNA expression (0.134 ± 0.037 )was significantly decreased compared with single-drug,zoledronic acid ( 0.208 ± 0.040 ) and cisplatin (0.356 ± 0.033) ( P ＜ 0.05 ).Conclusion Zoledronic acid or cisplatin can significantly inhibit A549 cell proliferation and markedly induce the apoptosis.Zoledronic acid and cisplatin in a synergistic way inhibited the cell proliferation and induced apoptosis on A549 cell.The downregulated expression level of MDC1 mRNA may involved in the mechanism of synergistic effect. Key words: Lung Cancer; Cisplatin; Zoledronic acid; A549 cell line; MDC1