Mechanism of apoptosis induced by Resveratrol in human gastric cancer HGC27 cells

Abstract

Objective To investigate the relationship between the expression of VEGF and MMP-9 in PTEN and p-Akt signaling pathway of resveratrol affecting gastric cancer HGC27 cells. Methods The effects of resveratrol on growth inhibition rate and cell cycle of HGC27 cells were examined by CCK-8 test and flow cytometry,respectively.The effects of resveratrol on apoptosis-related PTEN and p-Akt gene in HGC27 cells were detected by Western blotting,and the contents of VEGF and MMP-9 protein in HGC27 cells before and after resveratrol treatment were determined by ELISA. Results The growth inhibition rate of HGC27 cells by resveratrol treatment was concentration-and time-dependent(P0.05).Forty-eight hours after resveratrol treatment,cell cycle analysis indicated that the percent of HGC27 cells with DNA synthesis(S phase) significantly decreased,which exhibited a negative correlation with dosage(r=-0.930,P=0.007).There was a positive relationship between the relative expression of PTEN and Res concentration(r=0.951,P=0.04),while there was a negative relationship between the relative expression of p-Akt,VEGF and MMP-9 and Res concentration(r=-0.969,P=0.03;r=-0.894,P=0.02;r=-0.881,P=0.01). Conclusion Resveratrol could inhibit the proliferation and induce apoptosis of human gastric cancer HGC27 cells,which may be related to the expression up-regulation of PTEN and phosphorylation inhibition of Akt.Resveratrol could also regulate the expression of VEGF and MMP-9 to inhibit the proliferation and apoptosis of HGC27 cells.