Abstract
Mechanism of action of progesterone in the rabbit endometrium. Induction of uteroglobin and its messenger RNA.
Highlights
Uteroglobin and induced in the rabbit endometrium under the effect of prouteroglobin mRNA
Itthus becomes possible to study the molecular mechanisms of the induction by progesterone of a specific proteinand of its messenger RNA in the endometrium
Estradiol increased in the same proportion uteroglobin and its messenger RNA,whereas progesterone increased the uteroglobin concentration 3
Summary
(Receivedfor publication, September 19, 1980,and in revised form, December 30, 1980). Uteroglobin (measured by radioimmunoassay) and induced in the rabbit endometrium under the effect of prouteroglobin mRNA Decreased but did not entireIy suppress the effect of Radioimmunoassay of Uteroglo+n--Each uterine horn was rinsed progesterone on uteroglobin messenger RNA accumu- once with 1 ml of 0.01 M sodium phosphate, 0.15 M sodium chloride, lation. Administration of progesterone and/or estradiol for 6 days had different effects on the concentrations of uterogIobin and its messenger RNA in the endometrium. Hybridization to Complementary DNA-Preparation and characterization of DNA complementary to uteroglobin mRNA and hybridization conditions have been described [7, 8] This [“HIcDNA has a specific activity of1.71 X 10’ cpm/pg, a size of500600 nucleotides. The biological methods most widely used tostudyand measure the action of progesterone and progestins are those and hybridizes to pure uteroglobin messenger RNA with a rot1,*of 6.10“.
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