Abstract
Salmonella enterica serovar Enteritidis (S. Enteritidis) is one of the most important causes of foodborne illness in the food industry. In this work, plasma-activated water (PAW) generated from the plasma jet was employed to reduce S. Enteritidis contamination. The antibacterial mechanism of PAW was revealed from biological changes, molecular docking, and transcriptomics. Results revealed that the addition of lactic acid could improve the antibacterial activity and efficiency of PAW, and confirmed the existence and antibacterial activity of free radicals (OH, 1O2, and NO) in PAW. Meanwhile, PAW treatment caused damage to characteristic functional groups of biomolecules, and led to the oxidation of lipids and degradation of DNA. Molecular docking results further suggested that the reactive species could interact with amino acid residues around key binding sites through hydrogen bonding, affecting the stability of structures. Furthermore, the transcriptomics results indicated that PAW is primarily active on bacterial proteins and to a lesser extent on membrane lipids and DNA. Overall, the action mechanism of PAW on biomacromolecules is comprehensively analyzed in this work, and it provides a theoretical basis for the broad application of plasma technology.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
