Abstract
Objective To investigate the possible mechanism of 2-methoxyestradiol reversing multidrug resistance of K562/AO2 cells.Methods The inhibitory effects of 2-methoxyestradiol on the proliferation of K562 and K562/AO2 cells were observed by MTT assay.Annexin V-FITC /PI staining was used to detect apoptosis.Results The resistance of K562/AO2 cells to ADR was 50 folds compared with K562 cells.The IC50 of ADR was evidently reduced by 2-methoxyestradiol.The reversal folds of 2-methoxyestradiol for K562/AO2 cells were 5.9.The result of Annexin-V /PI staining showed that rates of the apoptotic cells were 10.32%,21.56% and 16.45% respectively,which were remarkably higher than that of control(6.68%).Conclusion 2-methoxyestradiol has reverse effect on K562/AO2 cells,its possible mechanism might be related with apoptosis of K562/AO2 cells.
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