Mechanism for Transduction of the Ligand-Binding Signal in Heme-Based Gas Sensory Proteins Revealed by Resonance Raman Spectroscopy

Abstract

Gene analysis has revealed a variety of new heme-containing gas sensory proteins in organisms ranging from bacteria to mammals. These proteins are composed of sensor, communication, and functional domains. The sensor domain contains a heme that binds effector molecules such as NO, O2, or CO. Ligand binding by the sensor domain modulates the physiological role of the protein, such as DNA binding in the case of transcriptional factors or the catalytic reaction rate in the case of enzymes. This Account summarizes resonance Raman (RR) studies, including static and time-resolved measurements, which have enabled elucidation of the mechanisms by which binding of specific target molecule by the sensor domain is transduced to alteration of the functional domain. These studies have shown that signals can be conveyed from the heme to the functional domain via three different pathways: (i) a distal pathway, (ii) a proximal pathway, and (iii) a heme peripheral pathway.