Abstract
Antiplatelet actions of aqueous extract of onion were investigated in rat and human platelet. IC50values of onion extract for collagen-, thrombin-, arachidonic acid (AA)-induced aggregations and collagen-induced thromboxane A2(TXA2) formation were 0.17 ± 0.01, 0.23 + 0.03, 0.34 ± 0.02 and 0.12 ± 0.01 g/ml, respectively. [3H]-AA release induced by collagen (10 μg/ml) in rat platelet was decreased by onion compared to control (22.1 ± 2.13 and 5.2 ± 0.82% of total [3H]-AA incorporated, respectively). In fura-2 loaded platelets, the elevation of intracellular Ca2+concentration stimulated by collagen was inhibited by onion. Onion had no cytotoxic effect in platelet. Onion significantly inhibited TXA2synthase activity without influence on COX activity. Platelet aggregation induced by U46619, a stable TXA2mimetic, was inhibited by onion, indicating its antagonism for TXA2/PGH2receptor. These results suggest that the mechanism for antiplatelet effect of onion may, at least partly, involve AA release diminution, TXA2synthase inhibition and TXA2/PGH2receptor blockade.
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