Mechanism, electrochemistry and biotoxicity analysis of the biodegradation of sulfadiazine on Nickel(Ⅱ)/Manganese(Ⅱ)-modified graphite felt bioanode

Abstract

Sulfadiazine (SDZ) is one of the most representative sulfonamides antibiotics, and its biodegradation has become a research hotspot in recent years. The present study innovatively adopted a microbial fuel cells with a Nickel (Ⅱ) and Manganese (Ⅱ)-decorated graphite felt bioanode (Ni(Ⅱ)/Mn (Ⅱ)-MFCs) to remove SDZ. The results demonstrated that the Ni(Ⅱ)/Mn (Ⅱ)-MFCs exhibited improved electrochemical performance, with a higher power density (742.98 ± 58.33 mW/m2) compared to the control MFCs (678.34 ± 52.87 mW/m2), an overall lower anode potential, and a larger double layer area (cyclic voltammetry). After 5 months of operation, approximately 97.95% of 30 mg/L SDZ was degraded within 120 h, which was 11.46% higher than that of the control MFCs. Moreover, SDZ and its byproducts could be better mineralized in the Ni(Ⅱ)/Mn (Ⅱ)-MFCs than the control, and the biotoxicity of SDZ towards Escherichia coli and Vibro qinghaiensis sp. Q67 could be greatly decreased after treatment with the modified MFCs. Based on the metabolites, we hypothesized that the chemical reactions hydroxylation, ammoxidation, SO2-extrusion, sulfur-reduction, etc. played a significant role in SDZ biodegradation. A microbial community analysis revealed that Dechloromonas (2.37%), Denitratisoma (5.32%) and Lentimicrobium (26.35%) were the dominant functional microbes in the Ni(Ⅱ)/Mn (Ⅱ)-MFCs. This study may provide insights and a theoretical basis for the biodegradation of sulfonamides and thus may facilitate further investigations and relevant findings.