Abstract
Calcium phosphate bioceramics such as hydroxyapatite (HA) have widely been applied as scaffolds in bone tissue engineering because of high osteo-conductivity and biocompatibility. In the present study, continuous porous HA scaffold was fabricated using the template method. Human mesenchymal stem cells (hMSC) were then seeded into the HA scaffold up to four weeks to observe the proliferation behavior and variation of the compressive mechanical properties. The scaffold with hMSCs was also characterized by scanning electron microscopy (SEM). It was found that the compressive strength and elastic modulus tend to increase with increasing culture time due to proliferation and attachment of the cells. The HA scaffold was also found to be suitable for hMSC adhesion, spreading and proliferation. Moreover, improvement of cellular adhesion was achieved by introducing RGD (Arg-Gly-Asp) peptide into the HA scaffold. We can conclude that the HA scaffold provides good environmental conditions for hMSCs as an artificial extracellular matrix. It is also important to note that the cellular adhesion can be effectively improved by RGD.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.