Mechanical Synthesis of Nano Carrier Based Bio-formulation of Trichoderma harzianum and Their Bio-efficacy against Fusarium Wilt of Chick Pea

Abstract

The terms "nano science" and "nanotechnology" have grown in popularity in comparison to micron-sized particles because they are more durable, efficient and have a high surface-to-volume ratio. In this regard, an experiment was conducted to assess the efficiency of a nano carrier-based formulation of Trichoderma harzianum against the chickpea wilt pathogen Fusarium oxysporum f. sp. ciceris. Carrier materials such as talc, lignite and fly ash were reduced to nano size using a high-speed cryo ball mill and particle sizes were determined using a particle size analyzer, scanning electron microscope and x-ray diffraction. The results revealed all of the concentrations of nano carrier formulated (talc, lignite and fly ash) T. harzianum viz.,100, 200, 300, 400 and 500 ppm concentrations were found to effective in inhibiting the growth of the F. oxysporum f. sp. ciceris with 100 per cent mean mycelial inhibition except nano talc formulated Trichoderma which shows mycelial inhibition of 2.56 per cent at 50 ppm concentration.