Mechanical properties of the Papio anubis tympanic membrane: Change significantly from infancy to adulthood

Abstract

Mechanical properties of the tympanic membrane (TM) are important for studying the transfer function of the auditory system. However, nearly all reported human data are limited to adults because of the unavailability of temporal bones from children. In this study, we used the baboon (Papio anubis), a genetically close human relative, as a model to address the occurrence of age-dependent changes of the human TM. Forty-five baboon TMs were characterized in five age groups: <1 year, 1 to <2 years, 2 to <3 years, 3 to <5, and >5 years of age, comparable to human ages ranging from newborn to adult. The elastic properties of the baboon TMs were characterized by a micro-fringe projection technique. Volume displacement of the TM under quasi-static pressure was first determined from its micro-fringe pattern. Subsequently, these displacement values were used in a finite element model to derive mechanical properties. The Young's modulus of the baboon TM exhibited a modest decrease from 29.1 MPa to 26.0 MPa over the age groups. The average Young's modulus was ∼1.4 times higher than that of the adult human TM. This is the first time that age-related TM mechanical properties of high primate are reported. These new findings may help to explore the potential value of the baboon as a new primate model for future age-related hearing research on the normal and diseased ear.