Abstract
Catch bond, whose lifetime increases with applied tensile force, can often mediate rolling adhesion of cells in a hydrodynamic environment. However, the mechanical mechanism governing the kinetics of rolling adhesion of cells through catch-bond under shear flow is not yet clear. In this study, a mechanical model is proposed for catch-bond-mediated cell adhesion in shear flow. The stochastic reaction of bond formation and dissociation is described as a Markovian process, whereas the dynamic motion of cells follows classical analytical mechanics. The steady state of cells significantly depends on the shear rate of flow. The upper and lower critical shear rates required for cell detachment and attachment are extracted, respectively. When the shear rate increases from the lower threshold to the upper threshold, cell rolling became slower and more regular, implying the flow-enhanced adhesion phenomenon. Our results suggest that this flow-enhanced stability of rolling adhesion is attributed to the competition between stochastic reactions of bonds and dynamics of cell rolling, instead of force lengthening the lifetime of catch bonds, thereby challenging the current view in understanding the mechanism behind this flow-enhanced adhesion phenomenon. Moreover, the loading history of flow defining bistability of cell adhesion in shear flow is predicted. These theoretical predictions are verified by Monte Carlo simulations and are related to the experimental observations reported in literature.
Highlights
Cell adhering to one another or their extracellular matrix under hydrodynamic flow is a crucial issue in many basic physiological and pathological processes, such as immune response [1,2], tumor metastasis [3], and targeted delivery of therapeutics to tissues [4]
There is a process under loading force, the theoretical framework describing the kinetic reactions between counterintuitive observation that one type of bonds can live longer at higher receptors and ligands was initially established by Bell [7,8]
We extend our previous adhesion modelIntothe establish paper, we extend our previous model to establish mechanical based on energy mechanical description based adhesion on energy conservation in acell adhesiondescription by taking into account the conservation in cell adhesion by taking into account the stochastic reaction of catch bonds as well as stochastic reaction of catch bonds as well as hydrodynamic impact from shear flow
Summary
Cell adhering to one another or their extracellular matrix under hydrodynamic flow is a crucial issue in many basic physiological and pathological processes, such as immune response [1,2], tumor metastasis [3], and targeted delivery of therapeutics to tissues [4]. There is a process under loading force, the theoretical framework describing the kinetic reactions between counterintuitive observation that one type of bonds (so-called catch bonds) can live longer at higher receptors and ligands was initially established by Bell [7,8].
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
