MECHANICAL AND STRUCTURAL PROPERTIES OF COLLAGEN NANOFRIBROUS LAYERS UNDER SIMULATED BODY CONDITIONS

Jitka Říhová,Lukáš Horný,Monika Šupová,Lucie Vištejnová,Tomáš Suchý

doi:10.14311/app.2019.25.0073

Abstract

The theme of this paper is the analysis of mechanical and structural properties of nanoﬁbrous COL under simulated body conditions and in the presence of osteoblasts and dermal ﬁbroblasts. COL were prepared by electrostatic spinning of 8wt% collagen type I dispersion with 8wt% (to COL) of PEG in phosphate buﬀer/ethanol solution (1/1vol). The stability of COL was enhanced by means of cross-linking with EDC and NHS at a molar ratio of 4:1. COL were exposed in culture medium for 21 days and human SAOS-2 human dermal ﬁbroblasts and osteoblasts were cultured therein for 21 days as well. The cell culture on COL was assessed by ﬂuorescence microscopy and metabolic activity. Then the metabolic activity of both cell types grown on COL and PS were measured after 1, 7, 14 and 21 days using the Alamar Blue assay method. Mechanical properties were determined using an tensile test. The inﬂuence of the cell activity on secondary structure of COL was veriﬁed by IR spectroscopy. Furthermore, the inﬂuence of cells on COL was evaluated by SEM.

Highlights

Part of the tissue engineering is research and development of new materials which will be used in biomedicine
Biological test After the first day of culture, both dermal fibroblasts and osteoblasts were similar to the rounded shape of the cell on PS and on the Collagen nanolayers (COL)
During this work it was found that both fibroblasts and osteoblasts can influence the mechanical and structural properties of collagen layers by their activities

Summary

Introduction

Part of the tissue engineering is research and development of new materials which will be used in biomedicine. One of the directions is the development of biocompatible nanofibers which can be prepared, for example, from polymers. These nanostructures can provide temporary mechanical and structural support for cells and help restore damaged tissue. Current research in the field are concentrated mostly on materials that are completely resorbable in the human body over time and their properties can simulate the function of the original tissue. The term biodegradation has been known for degradation occurring in biological environment. Changes in the physico-chemical properties of materials that are exposed to body fluids are the result of chemical, physical, mechanical, and biological interactions between the material and the environment [5, 6]

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Conclusion