Abstract
In this paper, we propose a new and simple method based on two-photon excitation fluorescence (TPEF) microscopy to measure the scattering coefficient µ(s) of thick turbid media. We show, from Monte Carlo simulations, that µ(s) can be derived from the axial profile of the ratio of the TPEF signals epi-collected by the confocal and the non-descanned ports of a scanning microscope, independently of the anisotropy factor g and of the absorption coefficient µ(a) of the medium. The method is validated experimentally on tissue-mimicking optical phantoms, and is shown to have potential for imaging the scattering coefficient of heterogeneous media.
Highlights
Non-invasive measurement of the optical properties of tissues offers interesting possibilities and perspectives in terms of in vivo diagnosis and therapy [1]
Inhomogeneity and polarization effects, such media can be characterized by three optical parameters which are the scattering coefficient μs, the associated anisotropy factor g = and the absorption coefficient μa
A two-photon excitation fluorescence microscope based on an infrared (IR) coated confocal microscope (FV300/BX51WI, Olympus France, Rungis) and a femtosecond Ti:Sapphire laser (Verdi-V5/Mira, Coherent France, les Ulis) tuned at = 830nm were used in this study
Summary
Non-invasive measurement of the optical properties of tissues offers interesting possibilities and perspectives in terms of in vivo diagnosis and therapy [1]. Inhomogeneity and polarization effects, such media can be characterized by three optical parameters which are the scattering coefficient μs (the inverse of the mean free path ls between two scattering events), the associated anisotropy factor g = (the average cosine of the scattering angle ) and the absorption coefficient μa (μa
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