Abstract
This article presents a brief review of literature on the physical chemistry of lipid phase transitions with emphasis on their kinetic properties. The theoretical foundations of perturbation techniques, and specifically the volume-perturbation technique are discussed in some detail. These are presented as a rationale for, and introduction to, a volume-pertubation kinetic calorimeter that we have constructed for measurement of the kinetics of lipid phase transitions. The instrument has been applied to study the gel-liquid crystalline phase transition in a variety of phospholipid bilayer systems. The design and implementation of the volume-pertubation calorimeter are presented along with a discussion of the techniques of data analysis. Finally, we present typical results obtained with this methodology for a multilamellar vesicle dispersion of dipalmitoylphosphatidylcholine.
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