Abstract
Rates of nitrogen fixation were determined for alder (Abuts tenuifolia Nutt.) with the C2H2 assay and verified by the 15N method. Rates of C2H2 reduction obtained varied between 0.0342 and 10.9 μmoles per gram of nodule tissue (fresh weight) per hour with a seasonal average of 2.70 μmoles g-1 hr-1. Expressed as nitrogen fixation rates these are equivalent to 0.0114, 3.63, and 0.9 μmoles g-1 hr-1 of elemental nitrogen, respectively. Nitrogen fixation (15N) rates averaged 2.20 μmoles N g-1 hr-1 with a standard deviation of 1.14. The influence of pC2H2, pO2, temperature during incubation, length of incubation time, presence vs. absence of N2 in the incubation atmosphere, and amount of nodule biomass per sample were investigated.
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