Abstract
Nodulated legumes contribute significantly to the N economy of soils and, in so doing, increase agricultural yields of food crops (Dakora and Keya, 1997). The amount of N fixed in symbiotic legumes by root-nodule bacteria (species of Rhizobium, Bradyrhizobium, Allorhizobium, Agrorhizobium, Mesorhizobium and Sinorhizobium) has generally been assessed under field conditions using the N natural-abundance technique and/or the N difference method. Although the N natural-abundance technique has been used successfully for field measurements of N2 fixation in cultivated food grain and pasture legumes, the method is beset by problems associated with the choice of reference plants, especially in natural settings. When using this method, the N derived from atmospheric nitrogen fixation (%Ndfa or Pfix) is usually estimated (Shearer and Kohl, 1986) as:
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