Abstract
The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.
Highlights
The introduction of local resolution has enormously helped the understanding of cryo-EM maps
What we are doing is to treat local resolution not just as a single “value”, the directional resolution operator assigns a real number to every direction at every voxel, the changed is, of a very fundamental mathematical nature
MonoDir works by establishing statistical tests to elucidate if the local energy of signal is higher than the energy of noise
Summary
The introduction of local resolution has enormously helped the understanding of cryo-EM maps. The global one is a single parameter that determines the degree of reliability of the whole map; being the FSC the most common Recent works like the 3DFSC5 or the efficiency[6] have tried to address individually some of these problems introducing the concept of directional resolution These approaches were intrinsically global, determining a merit function that informs on the existence of a bad angular coverage. We will show here that, as opposed to just local resolution methods[2,3,4], by expanding the concept of (voxel) locality by performing a complete “directional local resolution” analysis, other way to analyze the informational content of a cryo-EM map emerges, opening many new possibilities, from map validation and quality control to chain tracing, among others. The indicator that we have introduced is “the Average
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