Abstract
Noninvasive techniques have been developed for the assessment of various parameters in normal and diseased tissues of mice. This protocol describes the measurement of extravascular parameters, including interstitial diffusion, convection, and binding parameters, in mouse tumors. A fluorescently labeled molecule of interest is infused into the tumor interstitium, followed by imaging using single-photon microscopy or multiphoton laser-scanning microscopy (MPLSM). Fluorescence recovery after photobleaching (FRAP) with spatial Fourier analysis is performed. To measure interstitial diffusion coefficients, multiphoton FRAP is performed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
