Abstract
This paper describes the role of various external redox mediators in biodetector systems using bioinspired nanomaterials based on magnetite@polydopamine (Fe3O4@PDA), and their influence on the working parameters of the designed systems. The investigated mediators were hydroxy(methylferrocene, 1,4-benzoquinone, methylene blue, hydroquinone, and [Fe(CN)6]3-/4- redox couple. The amperometric glucose biosensor was fabricated by immobilizing glucose oxidase from Aspergillus niger (GOx) onto the Fe3O4@PDA nanomaterial, with the addition of a strictly defined amount of a specific external mediator. Among all tested mediators, the most favorable results were obtained for hydroxymethyl(ferrocene) with the integrated GC/Fe3O4@PDA-GOx system. The mediator exhibited ahigh linear amperometric response in a glucose concentration range from 1 to 20 mM, with a relatively high sensitivity of 139.71 μAmM−1 cm−2 and a limit of detection of 1.54 μM. Moreover, the system showed good stability over time, reproducibility, and resistance to interference.
Published Version
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