Abstract
Abstract The nucleoside triphosphate (NTP) pool during the early development of sea-urchin, Paracentrotus lividus, was measured using high-performance liquid chromatography (HPLC). Each NTP pool was measured at four stages between the eight-blastomere stage and the early blastula stage, as was the specific activity of each NTP following an initial administration of 8- 3H-guanosine. Unexpectedly high UTP (uridine-5′-triphosphate) concentrations and radioactivity levels in UTP were observed. Studies of the sea-urchin, Strongilocentrotus purpuratus, also revealed elevated levels of 8- 3H-guanosine incorporation into UTP. The present procedure was found to be suitable for the unequivocal identification of UTP.
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