Abstract
Inverse Gas Chromatography was applied to study certain biophysical parameters related to structural characteristics of the DNA molecule. In addition, the same method was introduced to study the relationship between radiation dose and the destruction of hydrogen bonds between the chains of the DNA double helix. This destruction of hydrogen bonds in the region of nearby induced single strand breaks (SSB) of the DNA molecule has been associated with the appearance of double strand breaks. To this effect the Gibbs Free Energy, the Enthalpy, the Excess of Enthalpy and the Entropy of sorption between volatile and non volatile organic compounds and DNA molecule was studied in four different temperatures and six different γ-radiation doses. Isopropanol and Ethyl Acetate, capable of participating in H-bonding, exhibited the greatest adsorption propensity and reacted exothermically with the DNA molecule, while Toluene appeared to cause swelling of the DNA polymer.
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