Abstract
Serum testosterone, 11-ketotestosterone, and 17β-estradiol levels in the farmed great sturgeon (N = 14 for each sex; weight 3.7–4.4 kg; length 54–63 cm) were measured for potential use of steroid levels for identification of males and females. The fish were anesthetized in 300-L plastic containers using clove (Syzyglum aromaticum) oil at 200 ppm for about 10 min, and then blood was taken from the caudal vein. All individuals with testosterone level higher than 0.2 ng/mL were male as verified also by biopsy. 11-Ketotestosterone level in males was higher than that in females (P 0.05). The higher testosterone and 11-ketotestosterone levels in males allowed female individuals to be detected for further farming up to the stage IV of gonad development, i.e., caviar production and males for milt production.
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