Abstract
The electrode we have devised consists of a glass-insulated, recessed gold cathode with a tip of about 1 μm. The methods used in its construction, and that of a double-barrelled electrode, are described. To assess its validity for intracellular measurement of PO 2 we have used several tests, some of which are discussed in detail. The most unique consists of measuring the oxygen profile in freshly excised brain tissue or a yeast-agar mixture. A rearrangement of the classical diffusion equation to (P o − P) X ( P o = PO 2 at surface; P = PO 2 at known depths from the surface; X = depth from surface) plotted against X should give a straight line down to very low values of PO 2. In vivo measurements made with this electrode indicate that cell PO 2 normally fluctuates with time, and the mean cell PO 2 is usually lower than the PO 2 in the venous outflow.
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