Abstract
Allium cepa root tip squashes have been prepared with 3:1 alcohol-acetic acid, Benda and Kahle's fixatives, and Feulgen, Leishman and orcein stains. Microphotographs of metaphase and anaphase cells have been taken together with one of a stage micrometer to give the magnification. The shadow areas, lengths and widths of the chromosomes have been measured. The standard deviation of a single measurement was about 10 per cent irrespective of whether the cells were from different bulbs, from different roots on the same bulb, or all from one root. Different fixatives and also different stains with the same fixative gave significantly different results. Anaphase chromosomes were shorter and wider than metaphase chromatids. The volume of a chromatid set was calculated from the measurements with each combination of fixative and stain. The volume necessary to contain the known amount of nucleoprotein was also calculated and found to be within the range spanned by these measurements.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
