Abstract
Since migraine prevalence is 2-3 times higher in women than in men, especially during the reproductive years, fluctuations in female sex hormone levels seem to be one of the key factors involved in the pathogenesis of migraine. During the last decade, lots of animal research in migraine has been performed on mice, since this species is well suited to create transgenic animal models1. To investigate the effect of female sex hormones in a murine model, it is important to analyse the hormone levels and/or to determine the hormone cycle in mice. Although no reliable assay has been available to measure murine plasma estrogen, recently 17β-estradiol assays have been suggested to be able to quantify these hormone levels.
Highlights
Since migraine prevalence is 2-3 times higher in women than in men, especially during the reproductive years, fluctuations in female sex hormone levels seem to be one of the key factors involved in the pathogenesis of migraine
We set up a pilot study to test 3 different ELISA kits described in the literature
All the tested ELISA assays did not show any differences in 17b-estradiol levels before and after OVX
Summary
Since migraine prevalence is 2-3 times higher in women than in men, especially during the reproductive years, fluctuations in female sex hormone levels seem to be one of the key factors involved in the pathogenesis of migraine. Measurements of 17b-estradiol levels in mice for migraine research From The European Headache and Migraine Trust International Congress London, UK.
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