Abstract
Clinical analytes, which are used for early detection of disease, are rapidly deteriorated. Thus, the preserving the quality of such analytes is highly required. Preservation of clinical analytes with the room-temperature desiccation is an ideal preservation method, although controlling the moisture in a specimen with a glass forming bio protective substance, e.g. trehalose, is essential for the drying process to be successful. In this study, the temporal change of the water concentration distribution in the high concentration aqueous solution of protective agents during the drying process was measured by microscopic infrared spectroscopy using infrared absorption band specific to water. Based on the temporal change of the water concentration distribution, the water self-diffusion coefficient in the high concentration trehalose and ε-poly-L-lysine(PLL) aqueous solution was estimated.
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