Abstract
A relatively simple and rapid radioimmunoassay for the determination of aldosterone in urine has been developed, employing tritiated aldosterone and a commercially available antiserum of high titer. Chromatography of specimens prior to immunoassay has been found to be unnecessary. The method involves extraction of a 3 ml aliquot of urine with dichloromethane (DCM), followed by a 24-h acid hydrolysis and a second DCM extraction. Extracts are ready for immunoassay after filtration through silica gel. Half-saturation with ammonium sulphate is used to separate free from antibody-bound aldosterone. Recovery is 90%, between-run coefficient of variation is ±14%, blanks are negligible, and comparison with the double isotope derivative method show no statistical difference and the same normal range.
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