Measurement of Uncertainty Associated with Quantification of Ethephon

Abstract

Objectives: Focus of the Research experiment is to evaluate the uncertainty in Ethephon determination. Validation of analytical method and identify the factors effecting the uncertainty in measurement. Methods/Statistical Analysis: A Titrimetric Method was used for determination of Ethephon content. A certified commercially formulated solution of Ethephon 39.00 % (w/w) S.L has been taken as a sample for analysis. An estimation of uncertainty in quantification of Ethephon was determined by identifying the following parameters, uncertainty influencing factors and its contribution to measurement, Type A Uncertainty (UA), Type B Uncertainty (UB), Combined Uncertainty (UC), Expanded Uncertainty (UE) and uncertainty budget. An Experimentally calculated value of combined uncertainty in preparation of 0.1 N NaOH solutions is 0.132%. A value of relative standard uncertainty in repeatability test of Ethephon is 0.084%. Determined % bias value of test method is 0.06%. An estimated value of combined uncertainty in Ethephon determination is 0.373 %. Obtained value of expanded uncertainty with coverage factor of 2.26 with 95% confidence is 0.843%. Obtained % RSD of Ethephon test result is 0.26 %. LOQ value of analytical test method is 0.36%. A Measured uncertainty value in Ethephon determination is 39.06 ± 0.33%. Findings: This paper produces Technique for analytical method validation and uncertainty measurements in quantitative analysis of Ethephon. Application/Improvements: As per regulatory for laboratory accreditation bodies, it is mandatory to evaluate the uncertainty in measurement. Presented method provides extensive details regarding uncertainty measurements.