Abstract
The fluid dynamics method combined with a low-speed CMOS camera is used to measure the trap stiffness of holographic optical tweezers. In order to improve the accuracy of measurement, a piezoelectric platform is driven by multi-frequency sine-wave voltages to produce different fluid friction on a microsphere trapped by holographic optical tweezers. Experiments demonstrate that the method can effectively obtain the trap stiffness; with the increase of the laser output power, the trap stiffness rises linearly.
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