Abstract
A radioassay method for measuring the folate concentration of tissues is described. This procedure uses the folate-binding protein in beta-lactoglobulin, 3H-pteroylglutamatic acid and unlabeled 5-methyltetrahydrofolate as the standard and has a lower detection limit of 0.1 ng. The folate levels in rat liver determined by the radioassay were not significantly different from those measured by the microbiological assay using Lactobacillus casei as the test organism. Furthermore, deconjugation of the polyglutamyl folate compounds in the tissue was not necessary to measure the total folate pool by radioassay. This radioassay could prove to be useful in determining the folate content of small tissue samples and tissues containing antibiotics of folate antimetabolites.
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